Stelling will complete ten walking marathons in ten days between March 21 and 30.
The 250-mile route will lead the host of Soccer Saturday from the ground of his boyhood club, Hartlepool United, in Middlesbrough ground to Wembley Stadium.
Each day’s marathon begins and ends at a different football club and over the course of the walk will visit 31 clubs and stadia.
Russ Green, Hartlepool United chief executive, will be joining Stelling for the length of the walk.
Other famous friends and colleagues will join the pair for part of the way, including staff from Carlsberg who will walk with them from Northampton to Milton Keynes on March 28.
Carlsberg is a longstanding supporter of Prostate Cancer UK, which is also the official charity partner of The Football League.
Stelling said: “I deal in statistics – but the ones we are dealing with here are shocking. One man dies every hour from this disease: that’s six in the course of Soccer Saturday every week.
“I want to get football fans, and all men, learning about the risk and I want to help fund the research that can turn those statistics round.
“Part of my motivation is of course taking Hartlepool to Wembley. It’s a journey the club and its supporters have never made.
“I'm delighted that Carlsberg are backing the Men United March; with partners like them on board I think we're on the right track to making this disease something men and their loved ones no longer fear.
“To do this we need to raise vital funds – and I need everyone to support me and all the men affected by prostate cancer by visiting menunitedmarch.org and pledging their support.”
Paul Morris, sponsorship and activation manager at Carlsberg UK, said: “We are extremely proud of our on-going relationship with Prostate Cancer UK.
“It’s a fantastic cause that our people care deeply about and have supported for a number of years.
“We hope the association helps to amplify the organisation’s messaging to reach more men nationwide.”
Angela Culhane, chief executive of Prostate Cancer UK, said: “Jeff’s been a huge supporter of ours for many years, and has seen first hand the dangers of this disease through some of his closest friends in the business, and we are with him every step of the way on his epic Men United March.
“We are very grateful for the continued backing from Carlsberg, who have been friends of ours for several years. They, like us, are committed to fighting back against this disease.
“Also, by encouraging their staff to take part in the leg between Northampton and Milton Keynes, Carlsberg are a perfect example of what we call Men United; friends getting together and doing something great to beat prostate cancer.”
Culhane will be walking the day-four route between Rotherham and Chesterfield.
Prostate cancer kills 10,000 men in the UK every year. It is the most common cancer in men, and over 330,000 of them are currently living with the disease.