Leckford Estate Brut 2011 was made at English wine estate Ridgeview from Chardonnay, Pinot Noir and Pinot Meunier grapes and is to retail for £29.99.
It will be on sale in 27 branches of the supermarket, online and from the Leckford Farm Shop.
Waitrose is the first supermarket to plant a vineyard.
Its English wine buyer Rebecca Hull MW said: “We’re so excited to finally introduce the Leckford wine to our customers. It’s been more than four years in the making, with our dedicated partners at Leckford working hard to nurture the vines and harvest the grapes. The team at Ridgeview have done a fantastic job to produce an excellent quality English fizz to be proud of and we can’t wait for shoppers to try it.”