Four-packs of 50cl cans are being listed by branches of Morrisons and selected wholesalers in Scotland.
The beer was sold in the format in the off-trade until the late 1990s but has only been available in kegs in the on-trade since then.
The reintroduction of an off-trade version of Tartan followsthe acquisition of the Younger’s and McEwan’s brands by Wells & Young’s from Heineken in 2011.
Commercial director David Revell said: “When we took on the Younger’s brands we knew that there was something missing from Tartan.
“It still had a high level of brand awareness and continued to be popular with drinkers, but was only available in pubs.
“We wanted to give consumers the opportunity to enjoy their favourite brew wherever they are, which is why we have brought back the can.
“We are looking forward to rolling the product out across Scotland in the coming months.”