M&S Cambridgeshire Summer Ale is being produced by Oakham Ales and is available from this week until the first week in September.
The beer has an abv of 4.2% and will be available in 250 southern England stores and larger branches nationally.
The retail price will be £2.19 a bottle.
Oakham has recently won regional listings with Tesco and Waitrose for some of its bottled ale brands.
Managing director Adrian Posnett said: “It will introduce new drinkers to our beers that may not have had the opportunity to try our products on draught.”