A If you're intending to position yourself as an upmarket specialist wine shop, possibly not, but if you want to trade as a mainstream, mid-market off-licence, you may have a problem.
Harris International Marketing's SOLTrack research among shoppers, 51% of people who shop at off-licences are smokers, and 19% said that cigarettes were the main items they intended to buy. Just as importantly, smokers spend £6.33 more over the course of a week than non-smokers. That's about 33%.
When asked whether they would shop elsewhere for alcohol if the off-licence they frequented did not sell
tobacco, 13% of smokers said they would.
So by all means take a moral stand if you must - but bear in mind it's a decision which will almost certainly restrict profits and customer numbers, and you'll probably get sick to the back teeth of explaining to people that you don't sell cigarettes.