The measure was defeated by 54 votes to 49 in a debate on the ruling SNP’s Alcohol Bill which is passing through the Scottish Parliament.
Health minister Nicola Sturgeon will be asked to remove the minimum pricing proposal from the bill to allow the rest of it to complete its journey on to the statute books.
Scottish Tory health spokesperson Murdo Fraser was quoted by the Daily Telegraph website as saying it was “the end of the road for minimum pricing”.
He added: “It’s time for the SNP to put its plans on hold and sit down and work with the UK government and opposition parties on a joint approach which will have widespread political support.”
Richard Simpson, Scottish Labour public health spokesman said minimum pricing had threatened to see Scotland used as “an experimental laboratory”.